Analyzing the Carotenoid Composition of Melilot (Melilotus officinalis (L.) Pall.) Extracts and the Effects of Isolated (All-E)-lutein-5,6-epoxide on Primary Sensory Neurons and Macrophages

Melilotus officinalis is known to contain several types of secondary metabolites. In contrast, the carotenoid composition this medicinal plant has not been investigated, although it may also contribute biological activities drug, such as anti-inflammatory effects. Therefore, study focuses on isolation and identification carotenoids from Meliloti herba effect isolated (all-E)-lutein 5,6-epoxide primary sensory neurons macrophages involved in nociception, well neurogenic non-neurogenic inflammatory processes. The extracts was analyzed by high performance liquid chromatography (HPLC). main column (CLC) identified MS NMR. water-soluble lutein 5,6-epoxide-RAMEB (randomly methylated-?-cyclodextrin) investigated Ca2+-influx rat induced activation transient receptor potential ankyrin 1 agonist mustard-oil endotoxin-induced IL-1? release mouse peritoneal macrophages. (all-E)-Lutein significantly decreased percent responsive compared vehicle-treated stimulated control. Furthermore, endotoxin-evoked 100 µM form decreases macrophages, which opens perspectives for its analgesic applications.